Established in the year 2000 by the Department of Cell Biology and Molecular Genetics, the Imaging Core was designed to enhance research and education at the University by providing students and faculty with access to sophisticated light microscopes and imaging instrumentation whose purchase and maintenance costs far exceed the budgets of individual investigators. Serving as the primary resource for advanced light microscopy in the Biological Sciences at the University of Maryland, the mission of the facility is to to enhance research and education by
- Providing access to state-of-the‐art light microscopy and imaging instrumentation,
- Offering detailed training opportunities and support in basic and advanced light microscopy techniques and,
- Keeping researchers up to date with the latest technology and innovations in light microscopy.
Hours of Operation
Fully trained, independent users may access the instrumentation 24 hours a day, 7 days a week.
The Director is available to assist and train users from 8am-4:30pm Monday through Friday.
Services and Technologies Offered
The Imaging Core provides researchers with the opportunity to image live, fixed, unlabeled or fluorescently labeled samples using one of several available microscopes, including a Zeiss LSM710 confocal, a Leica SP5X confocal, and an Axiophot Fluorescence microscope.
Services include training on all equipment, guidance on experimental design and Director-assisted microscope operation.
Zeiss LSM710 Confocal microscope: This inverted microscope is equipped with the following lasers: 405 diode, Argon (458, 488, 514), 561 and 633 lasers. Objective lenses include 10x dry, 40x oil, 63x oil, 100x oil, 63x water. It has 3 spectral detectors plus a transmitted light PMT.
Leica SP5X Confocal microscope: This inverted microscope is equipped with the following lasers: 405 diode, Argon (458, 488, 514), white light laser (470-670nm in 1nm increments). Objective lenses include 10x dry, 20x multi-immersion, 40x oil, 63x oil, 63x water, 63x glycerol. It has 5 spectral detectors plus a transmitted light PMT. It has a motorized x-y stage, galvo-z stage, resonant scanner and environmental chamber.
Zeiss Axiophot: is an upright fluorescence microscope with standard DAPI, FITC, TRITC filters and digital camera.
The current rate schedule can be found here: https://biosciencecores.umd.edu/rates1.html